Background:

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

Applications:

WB, IHC, IF

Name of antibody:

MDM2 (phospho-Ser166)

Immunogen:

Synthetic peptide of human MDM2 (phospho-Ser166)

Full name:

MDM2 oncogene, E3 ubiquitin protein ligase (phospho-Ser166)

Synonyms：

HDMX; hdm2; ACTFS

SwissProt:

Q00987

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

90 kDa

WB Positive control:

293 cells treated with Hydroxyurea

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200